The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DNMT3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DNMT3B....

Murine DNA methyltransferase 3beta (DNMT3B) (GenBank Accession No. NM_001003961), amino acids 2-859 (end), with N-terminal His-tag and C-terminal FLAG-tag, MW = 99 kDa, expressed in Sf9 cells via a Baculovirus expression system.

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

DNA Methyltransferase 3B (DNMT3B) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of mouse DNMT3B in tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/ml, Sensitivity: 0.047ng/ml, Storage and Stability: Desiccation recommended. The...